zebrafish toxicity assay

Embryonic and larval Danio rerio (zebrafish) is increasingly used as a toxicological model to conduct rapid in vivo tests and developmental toxicity assays; the zebrafish features high genetic homology to mammals, robust, phenotypes, high-throughput genetic and chemical screening have made it a powerful tool to evaluate in vivo toxicity. Abstract. Optimization of the Zebrafish Embryo Developmental Toxicity Assay (ZEDTA) M.Tobor-Kaplon, D. van den Oetelaar, M. Beekhuijzen, H. Emmen, E. van de Waart Charles River, Den Bosch, The Netherlands 1 Introduction The ZEDTA is a promising and innovative method with a potential to replace the screening of teratogenicity in animals Plates were monitored for survival after 1-and 24-hour treatments. The protocol deals with exposing zebrafish embryos to a range of compound concentrations at 28°C throughout organogenesis, i.e. In recent years, the zebrafish embryo has increasingly attracted the attention of chemists and pharmacologists for its utility in identifying chemicals with pharmacological activity in a whole … Because of these advantages, zebrafish bioassays are cheaper and faster than mouse assays, and are suitable for large-scale drug screening. New … The zebrafish embryo model is already accepted as a validated alternative assay to assess fish acute toxicity (OECD, No. Lantz‐McPeak et al. (Sipes et al, 2011) • Future Directions The Zebrafish Embryo Toxicity (ZET) model is increasingly recognized as a valuable toxicity testing platform. from the gastrulation stage … Although first used experimentally for the genetic analysis of vertebrate development and neurobiology, the zebrafish has been adapted as a model for many human diseases. Zebrafish embryos at 4, 28 and 52 hpf were used to test potential toxic effects of pleurocidins in 96-well plates. In addition, non-zebrafish cell lines such as COS-7 cells transfected with different zebrafish AHRs, ARNTs, and a dioxin response element-driven luciferase reporter have been used to investigate different halogenated aromatic hydrocarbons for AHR agonist activity in transactivation assays (Andreasen et al., 2002a; Tanguay et al., 1999). Zebrafish embryo toxicity assay. In view of safety of pregnant women, a promising in vitro zebrafish embryo developmental toxicity assay has been developed to test pharmaceutical and chemical compounds for their teratogenic potential. Larval zebrafish assay has excellent reproducibility even with n=2- 3. One of the biggest challenges in zebrafish embryo toxicity assay is the difficulty in maintaining concentrations and often the actual exposure concentrations can be significantly lower than the concentration used in the test . the zebrafish embryo toxicity and teratogenicity assay Jordan Ferdin A. Halili and Jonas P. Quilang Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon • Good correlation between single high dose study and dose response study. The zebrafish embryo model is widely recognized as a potential new approach method for chemical testing that may provide a bridge between cell and protein-based assays and mammalian testing. Here we describe the use of zebrafish bioassays for assessing toxicity, angiogenesis, and apoptosis. ( 2015 ) performed high content screening of zebrafish embryos to examine the developmental toxicity of ethanol, nicotine, ketamine, and caffeine. • Larval zebrafish assay may correlate with mammalian assays, but it won’t be simple. Each peptide was tested on ten embryos at 0, 1, 5 and 25 μM in 100 μl of E3 medium, in triplicate. Toxicity Screening with Zebrafish Assay EPA Grant Number: R835168 Title: Toxicity Screening with Zebrafish Assay Investigators: Tanguay, Robyn L. Institution: Oregon State University EPA Project Officer: Klieforth, Barbara I Project Period: May 1, 2012 through April 30, 2016 Project Amount: $1,199,999 RFA: Developing High-Throughput Assays for Predictive Modeling of … • Larval zebrafish have metabolic capability. Image‐based high content assays for toxicity screening using transgenic zebrafish embryos with morphology and behavioral endpoints are also gaining popularity. Nicotine, ketamine, and are suitable for large-scale drug zebrafish toxicity assay n=2-.. 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