phenol red indicator

The phenol red dye indicator is a weak organic acid, and its unionized acid and base forms are present in a concentration ratio that is determined by the ionization constant of the acid and the pH of the medium according to the familiar Henderson-Hasselbach equation. Typically a change in color from red to yellow indicates high rates of… Phenol red, pH indicator (CAS 143-74-8) (ab146336) | Abcam ���ɨ#�3#P[ƶ�.K�-CV�3v$N$p B�@�] �e8: �yP;P��C�Q?y��P�1 ����o�z�iЯ.����y�`�,�8{�m-b��yR!�7�� (�$ˁb9�� b�1���A�}(�#L���G�3P2�����2���~y�Yv�Ғ�w���HE�d�^����{L!�&r˜�A�0�KB��w�IF �9�KC���+ `/��w�"V)��N�؁ AvHv��j��.ĺ��45��g[/y���-�˽����z�`��#�*�{�r��c�n��ѐ v0&� ,a+Q��\� �B�p`�h��X`�8xk�G6�+&�M���h � ���ܬ��'�y� ����@b7��hYG��ؠ��,h��mR��m3�2@ύ��� ��@��Ɓ!�E�@�� �-���($`�9#@�pM�M�*�\�4��`�R����. [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. Phenol red exists as a red crystal that is stable in air. Polio (IPV – Ipol) Next. Phenol red exists as a red crystal that is stable in air. DTXSID2044420. ,"]���n����S��^���`�*Xv*� ��`[5�f��U� q �[����-���Y�d�L�]��@�I9H��?�� 2s�&00��L�g`ڵ � �� Its color exhibits a gradual transition from yellow (λmax = 443 nm[2]) to red (λmax = 570 nm[3]) over the pH range 6.8 to 8.2. DSSTox_GSID_44420. Phenol red is a weak estrogen mimic, and in cell cultures can enhance the growth of cells that express the estrogen receptor. This observed color change is because phenol red loses protons (and changes color) as the pH increases. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. CHEMBL3187327. Color range: pH 6.0-8.5. Chemical Formula: C 19 H 14 O 5 S Brand: OXFORD Phenol red exists as a red crystal that is stable in air. Phenol red CAS 143-74-8 indicator ACS - Find MSDS or SDS, a COA, data sheets and more information. ?���3��.�ߞ�9;����?���VF�{�����Ƌ�wgW?�����woN/$������ӫӋ������O^\������o��IU����/W��N~���.�� CV�?�1��ɫ���!��D\ͣ�@�pz�H9����'�s����i In crystalline form, and in solution under very ac… phenol red indicator. Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to 8.2. A solution of phenol red is used as a pH indicator: its color When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. Phenol Red is used as a pH indicator in cell culture applications. Cite. Even higher levels of bromine (>20 ppm) can result in the secondary conversion of bromophenol red to bromophenol blue with an even lower pKa, erroneously giving the impression that the water has an extremely high pH despite being dangerously low. (A table of other common chemical indicators is available in the article on pH indicators.). In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color. Depending on the intended cell type different mixtures of salts, amino acids, vitamins, buffers, carbohydrates, fatty acids, and supplements such as serum are used. [1] It is a weak acid with pKa = 8.00 at 20 °C (68 °F). [12], Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits. grade. �8�z0E00�1�2:+0(�{0�0IU1�jPO`d�R`�a���ʰ����QoC��5�8y��[)p]`�����ɰ��"%�Кp;��?�i��H�c�; e�bVf[(�@� Р!# Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria.It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose). Standardized for colorimetric pH determination. Above pH 8.2, phenol red turns a bright pink (fuchsia) color. In Vitro ADMET Laboratories, LLC. HY-D0169A. There are numerous formulations for in vitro cell propagation. Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). Phenol red indicator solution for pH test Use 5 drops for pH test Keep out of reach of children Available in 1-ounce size New (3) from $11.99 + FREE Shipping. More information about phenol red indicator in vaccines coming soon. endstream endobj startxref Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. 16th Jan, 2015. h�bbd```b``>"@$S�T� "Y�"���`�"0��^ �"�R@$W&X6,k fk�H�H)} [9] It was the first test of kidney function and was used for almost a century but is now obsolete. Material Safety Data Sheet or SDS for Phenol red 107241 from MilliporeSigma for download or viewing in the browser. [1][7] However, this cyclic structure could not be confirmed by X-ray crystallography. Phenol Red sodium salt, ACS reagent. use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue–culture system 1 2 Specification Test Results. Phenol Red, sodium salt. [11] It has been used to induce ovarian epithelial cells from post-menopausal women to differentiate into cells with properties of oocytes (eggs), with potential implications for both fertility treatment and stem cell research. endstream endobj 14 0 obj <> endobj 15 0 obj <>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Rotate 0/TrimBox[0 0 595.275 841.889]/Type/Page>> endobj 16 0 obj <>stream This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). �]���{̧$�S"�X�)�S�L`ͥt����2�k0H9N���uE��$�Rt� u��b �-�R�q I�EA�rwL��5� �@c��Q����L8��hY�r���ɱ-�H��#s�1=c1 ��X�ao1�5���d)�@�v��ɣ���<9��������s��ɣ�(���O�������G�]��2��jϿ������+�J�ļ�O���Z� [6], In several sources, the structure of phenol red is shown with the sulfur atom being part of a cyclic group, similar to the structure of phenolphthalein. h��[k����+��F"l�M�=,Kε�H���6��Y`�5�u�����T�=�=3;;�R^��f�XUdUž�h:Ӈ.����E��/ߙ������r����d��+(�}��KΡK��>�.Y��"�.�T����R�P �Cz���DH]ʜ�Gm�GS��#�3z��R`�JE�cJ}� L���&c&l�2zN�!���z�}�]A��t=g���\ �g���_p�����zq���D_�2������{�������볷W�W�}~������W�~����˿�}~���_~�8{�⮗����خ��O�8�� @N���/O����΀���=����?�������Y �:��l��ǧo�/~����o�g�. [13], Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. Phenol Red pH indicator Synonym: Phenolsulfonphthalein CAS Number 143-74-8. Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. Shop a large selection of Benzofurans products and learn more about Phenol Red, Pure, Indicator Grade, Fisher Chemical™: Benzofurans Organoheterocyclic compounds . Phenol red is yellow at a pH < 6.8 and red at a pH of > 7.4, therefore if a bacterium ferments a sugar to acid a yellow color will develop. Positive: After incubation the liquid in the tube turns yellow (indicated by the change in the color of the phenol red indicator). High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). EC Number 205-609-7. Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories. That I am using is " Phenol Red indicator, ACS reagent, CAS Number 143-74-8 ". Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories. %PDF-1.6 %���� h�b``�a``�b 'P�0p4p ��@1C � �(��u February 28, 2019 admin Ingredients 0. MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. 2. Phenol Red Indicator . Indicators usually exhibit intermediate colors at pH values inside the listed transition range. PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. Empirical Formula (Hill Notation) C 19 H 14 O 5 S . Typically, 15 mg/l are used for cell culture. Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. KS-00000XAO. While its presence is not critical for maintaining cell cultures, it is often used as a quick means for researchers to check on culture stock… Phenol red is a pH indicator. 126 0 obj <>stream DSSTox_CID_24420. pH indicators generally change color over a range of two pH units. Phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimicking actions. Phenol Red Indicator Solution. MDL number MFCD00003552 Bottle/50 mL spec. 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