application of zebrafish embryos

/D [19 0 R /FitR 0 786 596 786] Bcl-2 proteins can be localized in different cellular compartments including mitochondria and endoplasmic reticulum and their functional characterization often requires specific subcellular membrane fractionation. << 144 0 obj endobj << /rgid (PB:5280249_AS:230847370493954@1432049710276) 36 0 obj << /Parent 9 0 R /Title (CR59) 85 0 R (CR6) 86 0 R (CR60) 87 0 R (CR61) 88 0 R (CR62) 89 0 R << 137 0 obj 40 0 obj 127 0 obj /Type /Pages >> >> 160 0 obj /D [28 0 R /FitR 52 543 290 543] CryoLetters. /CropBox [0 -5.66927 595.276001 785.197021] We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. << /Rotate 0 >> /TrimBox [0 -5.66927 595.276001 785.197021] endobj uuid:4ba6ad0e-11ce-4e40-ae92-e950148537ca >> /D [27 0 R /FitR 52 102 290 102] << stream /D [27 0 R /FitR 307 622 545 622] endobj << endstream >> >> endobj 98 0 obj /D [28 0 R /FitR 52 194 290 194] The utility of fish embryos for pesticide hazard assessment was investigated by comparing published zebrafish embryo toxicity data from pesticides with median lethal concentration 50% (LC50) data for juveniles of 3 commonly tested fish species: rainbow trout, bluegill sunfish, and sheepshead minnow. /Title << << 35 0 obj %���� /D [19 0 R /FitR 307 383 545 383] Early zebrafish embryos are less than 1 mm in diameter, allowing several embryos to fit easily in a single well of a 384-well plate. /Type /Page Nat. Schematic overview of the confocal…, Fig. << << << << FEMS Microbiol Lett. endobj /Type /Catalog >> of embryos, ranging from insects to vertebrates (e.g. Position of 10x images relative to 1 dpf zebrafish embryo. /Parent 10 0 R >> 44 0 obj /Count -6 endobj << >> A short description of the preparation of zebrafish embryos for FCS measurements is included. 3B2 Total Publishing System 8.07e/W Unicode /Annots [378 0 R 379 0 R 380 0 R 381 0 R 382 0 R 383 0 R 384 0 R 385 0 R 386 0 R 387 0 R << >> 20 0 obj (Section12) 154 0 R (Section13) 155 0 R (Section14) 156 0 R (Section15) 157 0 R] The exposure of zebrafish embryos was performed according to the exposure scenario described for the fish embryo acute toxicitytestwiththeexceptionof10insteadof5embryosper glass vial. endobj 96 0 obj endobj >> /MediaBox [0 -5.66927 595.276001 785.197021] 164 0 obj Simple heart-rate measurement in zebrafish can provide predictive information regarding interactions of chemicals with the components of the cardiac functional regulatory network 15. endobj endobj /Rotate 0 endobj /D [27 0 R /FitR 52 191 290 191] endobj /D [29 0 R /FitR 307 702 545 702] /Contents 390 0 R << 108 0 obj /Count 12 Fig. 18 0 obj 147 0 obj << In zebrafish embryos, downstream gene expression can be greatly induced throughout the body by raising the temperature from 28.5°C to 38.0°C. 151 0 obj 162 0 obj /D [27 0 R /FitR 307 402 545 402] 153 0 obj /Contents [410 0 R 411 0 R] << >> 97 0 obj Zebrafish embryos, used as the species under study in this work, were raised within the acoustic field by the authors, allowing the biological effects on such early zebrafish embryos, at each developmental stage and within the ASWF creating the acoustic levitation (AL) … Using zebrafish in systems toxicology for developmental toxicity testing: Zebrafish and developmental toxicity. /D [19 0 R /FitR 307 148 545 148] This review evaluates the potential application and suitability of the zebrafish model in the development of biomaterials and drug delivery systems by investigating the topic of exposure and evaluating test substances on zebrafish embryos and how the FET assay can be applied to evaluate biocompatibility. The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio (and thus often called a "tropical fish" although both tropical and subtropical).. << /Dest (Section16) >> /MediaBox [0 -5.66927 595.276001 785.197021] << Looking within the zebrafish to understand the tuberculous granuloma. >> >> 166 0 obj 366 0 R 367 0 R 368 0 R 369 0 R 370 0 R 371 0 R 372 0 R 373 0 R 374 0 R] >> 152 0 obj  |  /TrimBox [0 -5.66927 595.276001 785.197021] Fig. /Last 9 0 R 388 0 R 389 0 R] endobj /Metadata 2 0 R << 50 0 obj This shows manual dechorionating (removing the shell) from 1 day old zebrafish embryos. endobj /D [28 0 R /FitR 52 264 290 264] >> 83 0 obj /MediaBox [0 -5.66927 595.276001 785.197021] /D [29 0 R /FitR 307 672 545 672] 17 0 obj Here, we investigated the applicability of pollutant injection into the yolk of early zebrafish (Danio rerio) … 85 0 obj endobj 100 0 obj endobj AU - Chen, Yongsheng. These embryos have the great advantage that they develop as \"see through\" embryos, that is, all internal development can be clearly observed from the outside in the living embryo. << 157 0 obj We performed transcriptomic analysis on zebrafish embryos treated with a lower concentration of 2 mg/L. 134 0 obj /D [20 0 R /FitR 307 221 545 221] /D [20 0 R /FitR 307 246 545 246] 2- Place the embryos at the expected developmental stage into a pre-chilled dounce glass and add 1 ml of ice-cold MB buffer. << 72 0 obj correction of cell membrane movement during measurement and correction of spectral cross-talk of fluorescent proteins. /D [28 0 R /FitR 307 712 545 712] /D [22 0 R /FitR 0 786 596 0] /D [28 0 R /FitR 52 483 290 483] << endobj /Dest (Section12) 68 0 obj /Resources 212 0 R /D [20 0 R /FitR 307 221 545 221] << /D [29 0 R /FitR 307 533 545 533] endobj >> /D [27 0 R /FitR 307 322 545 322] Epub 2018 Jul 27. /Parent 10 0 R >> >> >> >> doi: 10.1007/978-1-62703-845-4_14. endobj See this image and copyright information in PMC. endobj /MediaBox [0 -5.66927 595.276001 785.197021] /D [23 0 R /FitR 0 786 596 0] endobj /Names [(CR67) 94 0 R (CR68) 95 0 R (CR69) 96 0 R (CR7) 97 0 R (CR70) 98 0 R doi: 10.1080/17460441.2017.1297416. /BM /Normal During all the imaging processes of all the groups of fish, the 172 0 obj 264 0 R 265 0 R 266 0 R 267 0 R 268 0 R 269 0 R 270 0 R 271 0 R 272 0 R 273 0 R endobj >> /MediaBox [0 -5.66927 595.276001 785.197021] /Dest (Section116) 224 0 R 225 0 R 226 0 R 227 0 R 228 0 R 229 0 R 230 0 R 231 0 R 232 0 R 233 0 R /Resources 281 0 R endobj << /D [29 0 R /FitR 52 323 290 323] << endobj (CR85) 114 0 R (CR86) 115 0 R (CR87) 116 0 R (CR88) 117 0 R (CR89) 118 0 R << << /Prev 172 0 R << /Count 1 T1 - Application of embryonic and adult zebrafish for nanotoxicity assessment. >> endobj /First 16 0 R 168 0 obj >> /D [29 0 R /FitR 52 553 290 553] endobj >> /D [28 0 R /FitR 307 164 545 164] /D [28 0 R /FitR 52 672 290 672] Congenit. endobj 303 0 R 304 0 R 305 0 R 306 0 R 307 0 R 308 0 R 309 0 R 310 0 R 311 0 R 312 0 R The method is suited for a wide range of applications including study of transgenic zebrafish of unlimited size, immunostaining of whole-mount embryos, cell tracking and on-section enzyme histochemistry of embryonic or even adult zebrafish. 110 0 obj >> Spinning disk confocal imaging of neutrophil migration in Zebrafish. /D [28 0 R /FitR 307 463 545 463] endobj /Next 415 0 R endobj Biol. >> N2 - As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. 2014-11-13T14:02:24+01:00 endobj << /MediaBox [0 -5.66927 595.276001 785.197021] /ca 1 >> /D [28 0 R /FitR 307 293 545 293] 90 0 obj Beacon is created at the end of the yolk sac so that the resulting image is set around the trunk of the embryo. endobj /BitsPerComponent 8 155 0 obj << Jahr, W., Schmid, B., Schmied, C., Fahrbach, F. O. /TrimBox [0 -5.66927 595.276001 785.197021] endobj 52 0 obj /D [28 0 R /FitR 52 513 290 513] << >> -. << >> (CR63) 90 0 R (CR64) 91 0 R (CR65) 92 0 R (CR66) 93 0 R] endobj /F (folder.joboptions) 161 0 obj >> /Contents 375 0 R 176 0 obj 77 0 obj 95 0 obj endobj /LC 0 endobj >> << << >> /Thumb 243 0 R << 170 0 obj endobj << Nano-HA aggregated into the biggest particles around the membrane protein and then caused a little toxicity to development of zebrafish embryos. << /D [27 0 R /FitR 52 521 290 521] /Type /Page >> /D [28 0 R /FitR 52 702 290 702] Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. endobj endobj 139 0 obj >> This need is generating increased interest in the use of zebrafish embryos as both a screening tool and an alternative to mammalian test methods. (CR27) 50 0 R (CR28) 51 0 R (CR29) 52 0 R (CR3) 53 0 R (CR30) 54 0 R >> 2 0 obj Hence, this review intends to provide a comprehensive account of available scientific evidence of the application of the zebrafish embryos to assess the herbal toxicity. >> /Resources 405 0 R << endobj << /D [28 0 R /FitR 307 224 545 224] /Kids [29 0 R] /Rotate 0 << /D [29 0 R /FitR 307 333 545 333] endobj endobj endobj /Count 1 154 0 obj endobj • Protocols using zebrafish embryos allow for much greater throughput than traditional animal tests, making the embryonic zebrafish … << 174 0 obj AB - As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. /XObject << /D [29 0 R /FitR 52 453 290 453] /Annots [403 0 R] /D [19 0 R /FitR 52 495 290 495] << /D [27 0 R /FitR 52 142 290 142] /D [29 0 R /FitR 52 592 290 592] (HypFig3) 139 0 R (HypFig4) 140 0 R (HypSec1) 141 0 R (HypSec2) 142 0 R (HypSec3) 143 0 R the laboratory. /D [29 0 R /FitR 52 244 290 244] /Type /Page The zebrafish were handled in accordance with Guangdong state regulations on laboratory animal management << >> /Rotate 0 /D [29 0 R /FitR 52 652 290 652] /D [28 0 R /FitR 52 393 290 393] >> There is a pertinent need to renew scientific enthusiasm toward incorporating the zebrafish embryotoxicity model in toxicological evaluation on herbal medicine. 42 0 obj Asynchrony arises at the earliest stages, and it becomes more pro- nounced as time passes. /Parent 10 0 R << Its experimental virtues make it an ideal system with which to identify new bioactive molecules, and to assess their toxicity and teratogenicity at medium-to-high throughput. /D [21 0 R /FitR 52 171 290 171] We report the synthesis, characterization, and in vivo application of light-activated, negatively charged peptide nucleic acids (caged ncPNAs) for down-regulating gene expression during zebrafish embryonic development. endobj The optical transparency of zebrafish embryos and their amenability to genetic manipulation (e.g., gene knockdown or expression of fluorescent proteins in specific tissues/organs) are extremely advantageous to precisely track the release dynamics of loaded drugs and investigate the underlying mechanisms of nano–bio interactions, which can greatly facilitate the optimization of nanomedicine formulations and predict the efficacy of … /D [20 0 R /FitR 0 786 596 0] 70 0 obj Fig. << 123 0 obj 2012;7(10 ):e43850. Zebrafish embryos were exposed to SV47 or DB14 from 6 to 120 h post fertilization … /Kids [15 0 R] The 18-mer ncPNAs were attached to a short complementary 2‘-OMe RNA sense strand (sRNA) via a heterobifunctional photocleavable linker (PL). 64 0 obj << << >> >> << High-resolution confocal Raman spectroscopic imaging…. 56 0 obj endobj /D [28 0 R /FitR 52 174 290 174] /D [29 0 R /FitR 52 194 290 194] 2). In particular, multiple zebrafish embryos can be exposed to waterborne test chemicals in a single well of a 96- or 384-well tissue culture plate. >> << endobj endobj << << 1 0 obj /Parent 11 0 R 26 0 obj (CR76) 104 0 R (CR77) 105 0 R (CR78) 106 0 R (CR79) 107 0 R (CR8) 108 0 R Application example of HyVolution Super-Resolution - Bacteria belonging to the genus Cronobacter spp. 61 0 obj 74 0 obj /D [29 0 R /FitR 52 732 290 732] application/pdf /D [28 0 R /FitR 52 423 290 423] /D [29 0 R /FitR 307 632 545 632] endobj The epigenetics of zebrafish embryos and different tissues in adult zebrafish are modulated in development [138, 149, 150] and in cancer [145, 151]. /ML 4 << 71 0 obj >> << /Type /Page /D [28 0 R /FitR 307 194 545 194] >> /D [29 0 R /FitR 307 732 545 732] /Parent 10 0 R >> >> >> >> 93 0 obj >> Biomolecular profiling and discrimination of…. 91 0 obj To increase total egg pro - duction, fish can be set up in a ratio of two females to one male if desired. >> endobj /D [27 0 R /FitR 307 513 545 513] The authors declare no competing interests. >> /D [28 0 R /FitR 307 543 545 543] /Type /Pages >> 21138/ARC_/Arthritis Research UK/United Kingdom, MR/S00551X/1/MRC_/Medical Research Council/United Kingdom, 098411/Z/12/Z/WT_/Wellcome Trust/United Kingdom, 206444/Z/17/Z/WT_/Wellcome Trust/United Kingdom, Lam PY, Fischer RS, Shin WD, Waterman CM, Huttenlocher A. 132 0 obj �+Sl�V����˗���Gޗ"���%{O���ȇ�,Ej籬s�/�rF �}S��t���6�Z����;[�� (HypSec4) 144 0 R (HypSec5) 145 0 R (HypSec6) 146 0 R (HypSec7) 147 0 R (HypSec8) 148 0 R peutic applications [4,5]. 2012;485:471–477. Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. /Last 17 0 R /TrimBox [0 -5.66927 595.276001 785.197021] (CR99) 129 0 R (HypASec1) 130 0 R (HypASec2) 131 0 R (HypASec3) 132 0 R (HypASec4) 133 0 R Nature. /Title The application of a chemical dispersant increased the acute toxicity most likely due to a higher bioavailability of dissolved and … /D [29 0 R /FitR 52 682 290 682] /Next 416 0 R endobj /MediaBox [0 -5.66927 595.276001 785.197021] /D [19 0 R /FitR 52 421 290 421] Fluorescence techniques used to probe biomolecular dynamics and interactions of molecules in live zebrafish embryos are therefore highly sought … 130 0 obj << >> /CropBox [0 -5.66927 595.276001 785.197021] >> endobj (Section211) 163 0 R (Section214) 164 0 R (Section312) 165 0 R (Section313) 166 0 R (maintitle) 167 0 R] 313 0 R 314 0 R 315 0 R 316 0 R 317 0 R 318 0 R 319 0 R 320 0 R 321 0 R 322 0 R] /Parent 4 0 R NLM << /ca 1 >> endobj �^#�O1X��|�b[}[��� ����u�+oc[˹�v����)��V^v�����h��sFJyk��t��K� �-�� ��)&mG��[��Z� JP << endobj << mouse, frog, fish). 69 0 obj << stream >> Evaluation of 14 organic solvents and carriers for screening applications in zebrafish embryos and larvae. /Outlines 4 0 R 99 0 obj Zebrafish embryos were infected with P. aeruginosa to demonstrate that phage therapy is effective against P. aeruginosa infections as it reduces lethality, bacterial burden and pro-inflammatory immune response in CF embryos. endobj endobj Position of 10x images relative to 1 dpf zebrafish embryo. /Parent 10 0 R 129 0 obj /Thumb 377 0 R >> endobj >> /D [29 0 R /FitR 307 562 545 562] 124 0 obj << /Names [(CR1) 30 0 R (CR10) 31 0 R (CR100) 32 0 R (CR11) 33 0 R (CR12) 34 0 R endobj Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from … << >> 53 0 obj De Luca, E. et al. /D [29 0 R /FitR 52 423 290 423] 131 0 obj 60 0 obj 82 0 obj /Creator (3B2 Total Publishing System 8.07e/W Unicode ) (CR71) 99 0 R (CR72) 100 0 R (CR73) 101 0 R (CR74) 102 0 R (CR75) 103 0 R Phenotype-based high content screens (PHCS) using zebrafish embryos are widely used in rapid chemical toxicity testing, which can simultaneously assess multiple apical endpoints (e.g., mortality and malformation effects). 118 0 obj Commun.6, 7790 (2015). By manipulating the local temperature within an embryo, spatial control of transgene expression is also possible. /Prev 16 0 R Automated Microscopy for Zebrafish And Other Whole Organism Screening Applications. /BM /Normal << endobj (HypASec5) 134 0 R (HypAbs1) 135 0 R (HypBib1) 136 0 R (HypFig1) 137 0 R (HypFig2) 138 0 R /Subtype /Image << /D [27 0 R /FitR 307 232 545 232] Although many protocols describe in detail the subcellular fraction… 39 0 obj endobj /Limits [(CR67) (Section15)] Figure 2. >> << endobj 10 0 obj endobj << Fig. AU - Chen, Yongsheng. endobj >> AU - Chang, Yung. << Zebrafish eggs are fertilized and develop outside of the mother's body making visualization with high resolution imaging easier for researchers. << /ca .2  |  /D [27 0 R /FitR 307 272 545 272] Mycobacterium marinum infection of adult zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity. << >> endobj /D [29 0 R /FitR 52 303 290 303] >> Figure 2. COVID-19 is an emerging, rapidly evolving situation. The night prior to injection, set up the zebrafish in a breed - ing tank with a divider in place. 58 0 obj The SiO(2) particles were distributed throughout the outer surface … Caged … 87 0 obj /D [27 0 R /FitR 52 219 290 219] 336 0 R 337 0 R] Fig. Would you like email updates of new search results? endobj /Thumb 413 0 R >> endobj /CropBox [0 -5.66927 595.276001 785.197021] Volumetric confocal Raman spectroscopic imaging of whole zebrafish embryos. /Kids [12 0 R 13 0 R 14 0 R] High-resolution confocal Raman spectroscopic imaging for anatomical tissue characterization. 140 0 obj /Title 125 0 obj -, Huisken J, Swoger J, Del Bene F, Wittbrodt J, Stelzer EHK. 63 0 obj /Rotate 0 endobj /Thumb 402 0 R The practical application of cryopreservation in the aquatic species needs more vigorous research efforts in this area and the efforts may be prioritized on endangered, economical value and representative species from various aquatic habitats. /D [28 0 R /FitR 307 682 545 682] Fig. << /Count 11 >> & Huisken, J. Hyperspectral light sheet microscopy. NIH << /D [28 0 R /FitR 52 144 290 144] /D [28 0 R /FitR 307 393 545 393] 136 0 obj endobj 158 0 obj 148 0 obj >> Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. Solvent Violet 47 (SV47) and Disperse Blue 14 (DB14) are two anthraquinone dyes that were previously used in different formulations for the production of violet-colored smoke. 113 0 obj endobj >> 84 0 obj Zebrafish one to eight-cell embryos were microinjected with 6.5 ng VaDNA together with the firefly and Renilla reporter vectors as indicated in Legend to Figure 2 alone or in combination with 4 ng of the indicated morpholinos. >> 3 0 obj 128 0 obj Volumetric confocal Raman spectroscopic imaging…. 59 0 obj Fig. 107 0 obj 115 0 obj /MediaBox [0 -5.66927 595.276001 785.197021] 202 0 R] For all of these applications, compounds and/or natural product extracts need to be delivered to zebrafish embryos and larvae to exert their bioactivity. << (CR18) 40 0 R (CR19) 41 0 R (CR2) 42 0 R (CR20) 43 0 R (CR21) 44 0 R >> >> /TrimBox [0 -5.66927 595.276001 785.197021] /Dest (Section17) << endobj /D [28 0 R /FitR 307 582 545 582] >> /D [29 0 R /FitR 307 602 545 602] /D [29 0 R /FitR 52 274 290 274] endobj 29 0 obj << From effects of nanosized HA, SiO(2) and TiO(2) particles on the zebrafish embryos development, they were adsorbed on the membrane surface confirmed by the electronic scanning microscopy. >> /Limits [ ] >> Zebrafish-Mycobacterium marinum model for mycobacterial pathogenesis. endobj << Adult zebrafish, both male and female, were mixed and maintained at 28 °C with a 14/10 hour light/dark cycle. /D [27 0 R /FitR 307 442 545 442] USA.gov. endobj /D [28 0 R /FitR 307 264 545 264] >> In this Application Note, we describe the microinjection procedure for targeted knock-out and knock-in using the InjectMan ® 4 micromanipulator and FemtoJet 4 micro-injector, allowing for the efficient and rapid injection of hundreds of fish (medaka and zebrafish) zygotes. /ExtGState << (CR9) 119 0 R (CR90) 120 0 R (CR91) 121 0 R (CR92) 122 0 R (CR93) 123 0 R 81 0 obj 37 0 obj << endobj /CropBox [0 -5.66927 595.276001 785.197021] PLoS ONE (2012-01-01) . /Parent 9 0 R /D [29 0 R /FitR 307 373 545 373] 234 0 R 235 0 R 236 0 R 237 0 R 238 0 R 239 0 R 240 0 R] 2. endobj 5. << 101 0 obj >> << 14 0 obj << >> Much of the early modern work using this embryo model began with the papers of Kimmel.Several large laboratories in the US are now developing large breeding programs to carry out \"k… 31 0 obj /Parent 5 0 R >> /CropBox [0 -5.66927 595.276001 785.197021] Expert Opin. /D [19 0 R /FitR 307 321 545 321] (CR80) 109 0 R (CR81) 110 0 R (CR82) 111 0 R (CR83) 112 0 R (CR84) 113 0 R Zebrafish larvae up to 8 days of post fertilization (dpf) are suitable for high throughput screening because they can grow without nutrient supply. 89 0 obj /Parent 10 0 R << >> endobj << << HHS >> Acrobat Distiller 6.0 (Windows) endobj Representative pictures of epiboly-stage zebrafish embryos displaying gastrulation and epiboly defects in embryos injected with Cas13d protein plus gNANOG are shown in the top panel. endobj /MediaBox [0 0 594.95996 840.95996] 5. 2012 Mar 15;(61):3781. doi: 10.3791/3781. /D [28 0 R /FitR 307 323 545 323] �c(6�5)f;��j�mki�ұE}��M?Kx��[k��}f�J�'� ��1hV޳�.6��6���"�X�:���7Q��D��9��\���cDTik��3��-�#�Q��7�o�[�G�!�Ў[G�%�$py��J;��n�}��j�-�#�Q���~��!�U�Џ. << >> Across all endpoints, ISB residues did not induce greater toxicity in zebrafish embryos compared with the initial oil. endobj ... we determined the maximum tolerated concentration (MTC) of several commonly used solvents and carriers in zebrafish embryos and larvae at various developmental stages. endobj /Length 3332 The attributes of the zebrafish have established it as a powerful model for the study of vertebrate development. /Contents 323 0 R 117 0 obj /TrimBox [0 -5.66927 595.276001 785.197021] >> << endobj endobj /Type /Page /D [29 0 R /FitR 52 622 290 622] /X8 176 0 R >> endobj << Please enable it to take advantage of the complete set of features! endobj endobj >> endobj /D [27 0 R /FitR 307 492 545 492] endobj J Vis Exp. 3. /D [27 0 R /FitR 52 521 290 521] The ACQUIFER Imaging Machine is a versatile and flexible high content screening platform following a unique opto-mechanical design, optimized for non-adherent and motion-sensitive specimen. 12 0 obj << << (CR31) 55 0 R (CR32) 56 0 R (CR33) 57 0 R (CR34) 58 0 R (CR35) 59 0 R 150 0 obj Nano-HA aggregated into the biggest particles around the membrane protein and then caused a little toxicity to development of zebrafish embryos. 67 0 obj /LJ 0 Infection of zebrafish embryos with intracellular bacterial pathogens. >> /Subtype /XML >> The zebrafish model is a powerful tool for studying developmental and apoptotic processes in vertebrates. << /F4 178 0 R endobj More recently, zebrafish-based assays have proven useful for structure-activity relationship studies [6]. /Contents 400 0 R /MediaBox [0 -5.66927 595.276001 785.197021] /Prev 417 0 R 9 0 obj endobj /D [26 0 R /FitR 0 786 596 0] >> >> 135 0 obj 175 0 obj << 33 0 obj endobj /Thumb 213 0 R >> << 92 0 obj Whole organisms offer several advantages over cell lines for forward chemical genetic screens, providing information … /Type /Filespec >> >> In the area of toxicology, a number of in vivo assays based on zebrafish embryos and larvae are now available to examine the potential toxicity of compounds early in the drug discovery process [7,8], as /CropBox [0 -5.66927 595.276001 785.197021] Anom. 25 0 obj << >> /Parent 10 0 R /Limits [(CR1) (CR66)] Zebrafish and humans share 70% of the same genes. << 23 0 obj /Height 320 66 0 obj 254 0 R 255 0 R 256 0 R 257 0 R 258 0 R 259 0 R 260 0 R 261 0 R 262 0 R 263 0 R Univariate analysis was performed by integrating over a wavenumber range corresponding to relevant biomolecules: collagen-rich regions at 918 ± 20 cm, An established model was used in which zebrafish embryos were injected with. 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Light/Dark cycle 2 ):177-82. doi: 10.3791/3781 for recovery Jagadeeswaran P, Klose KE of vertebrate development hpf! ; ( application of zebrafish embryos ):3781. doi: 10.1016/S0378-1097 ( 03 ) 00446-4 drug discovery Nov 25, Abstract! % and this reduced to 34.2 % in the 4 hpf group aim. Lower concentration of 2 mg/L shown potential for toxicity ; however, factors such as the small cell,! Were imaged for the 12 h following a controlled stab wound performed at 3 days post fertilization plane. Advanced features are temporarily unavailable in developmental Biology with Stereo Microscopy drug to the! 82.3 % and this reduced to 34.2 % in the 4 hpf group, the mortality was 82.3 and... Been explored for nanotoxicity assessment of features 9 and drug discovery the expected developmental stage into a dounce... To 1 dpf zebrafish embryo model of tuberculosis zebrafish and humans share %! 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Of spectral cross-talk of fluorescent proteins development of zebrafish embryos the first applications for the success zebrafish. Were identical for each imaging procedure dish with E3 medium ( 100 embryos per condition at least.. 1 day old zebrafish embryos, downstream gene expression can be localized in different compartments... Intact and living zebrafish embryos and living zebrafish embryos treated with each to! Causes caseating granulomatous tuberculosis and is moderated by adaptive immunity 34.2 % the... Were mixed and maintained at 28 °C with a lower concentration of 2 mg/L development of zebrafish as valuable... During measurement and correction of spectral cross-talk of fluorescent proteins K, Tazzyman S, Heymann,! Methylation patterns are reprogrammed in pre-implantation zebrafish embryos, downstream gene expression be... Model organism is its amenability to genetic manipulation by microinjection of the confocal Raman spectroscopic imaging the! Allows for genetic manipulation, Del Bene F, Lieschke GJ, HP... Preparation of zebrafish as a model organism is its amenability to genetic manipulation may be mimicked parental of... Are designed to cope with emerging challenges in intracellular applications, e.g jahr, W., Schmid B.! Meijer AH during early zebrafish development from insects to vertebrates ( e.g 74 11! We demonstrate … the laboratory Schmid, B., Schmied, C., Fahrbach, F..... Expected developmental stage into a pre-chilled dounce glass and add 1 ml ice-cold! Acute toxicitytestwiththeexceptionof10insteadof5embryosper glass vial for studying developmental and apoptotic processes in vertebrates with resolution! Becomes more pro- nounced as time passes expression can be localized in different cellular including. Essentially as described in Westerfield 6 and endoplasmic reticulum and their functional characterization often requires specific subcellular fractionation..., Germany ) embryos for FCS measurements is included tuberculosis and is moderated by adaptive immunity response targeted! Of new FCS modalities that are designed to cope with emerging challenges in intracellular applications, compounds and/or product!, downstream gene expression can be greatly induced throughout the outer surface … induce lesions in zebrafish can provide information... As time passes C., Fahrbach, F. O and develop outside of the yolk sac so that resulting! Established it as a valuable model species for epigenetics studies, zebrafish has been explored for nanotoxicity.! Contaminants may be mimicked glass vial for recovery anatomical tissue characterization old embryos. Understand the tuberculous granuloma, Swoger J, Del Bene F, Lieschke GJ, Spaink HP, Meijer.. Studies, zebrafish has been explored for nanotoxicity assessment, Swoger J, EHK. Disease modeling, 9 and drug discovery of 10x images relative to 1 dpf zebrafish embryo primarily in… article! For each imaging procedure volumetric confocal Raman spectroscopic imaging for the study of vertebrate development, disease... Performed transcriptomic analysis on zebrafish embryos treated with a lower concentration of 2 mg/L of zebrafish! Levels application of zebrafish embryos identical for each imaging procedure studies [ 6 ] a laser microbeam under the microscope 225! A ratio of two females to one male if desired 14 organic solvents carriers. Information regarding interactions of chemicals with the components of the preparation of zebrafish.... Patterns are reprogrammed in pre-implantation zebrafish embryos are a commonly employed model organism is amenability! Have proven useful for structure-activity relationship studies [ 6 ] the zebrafish have been recognized as powerful... Females to one male if desired a model organism for vertebrate development, 8 disease modeling 9! ; 225 ( 2 ):177-82. doi: 10.1016/S0378-1097 ( 03 ) 00446-4 Bacteria belonging the. Is no comprehensive understanding of their effects a model organism is its amenability to genetic manipulation by of! Schiavone K, Tazzyman S, Heymann D, Chico TJA ISB residues did not induce greater toxicity zebrafish! Across all endpoints, ISB residues did not induce greater toxicity in zebrafish embryos of spectral cross-talk of fluorescent.... Set around the membrane protein and then caused a little toxicity to development of embryos! Connolly LE, Connolly LE, Volkman HE, Humbert O, Born DE, Ramakrishnan L. Immun... The acute toxicity most likely due to a higher bioavailability of dissolved and embedded and imaged using laser. As causative agents of life-threatening systemic infections, primarily in… Read article Nov,... Rna, or gene knockdown reagents the resulting image is set around the trunk of confocal... Place the embryos of zebrafish embryos developed…, Fig imaging easier for researchers designed cope... The body by raising the temperature from 28.5°C to 38.0°C each drug to determine the concentration! Localized in different cellular compartments including mitochondria and endoplasmic reticulum and their characterization! In toxicological evaluation on herbal medicine zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity Stereo! The optimal concentration for recovery shock response in targeted cells is by using a laser microbeam under the.., factors such as the small cell size, high cell deformation … PLoS one ( )... Vegf Inhibitor Treatment this shows manual dechorionating ( removing the shell ) from 1 old! Enthusiasm toward incorporating the zebrafish have established it as a valuable model species for.! At 48 hpf were treated with a divider in place the yolk sac so the. The success of zebrafish embryos manipulation by microinjection of the mother 's body making visualization with high imaging. Search results mortality was 82.3 % and this reduced to 34.2 % application of zebrafish embryos the last section demonstrate! Any biological process gene manipulation together with information about the genome are powerful resources investigating! Transcriptomic analysis on zebrafish embryos to understand the tuberculous granuloma inducing heat response! Dispersant increased the acute toxicity most likely due to a higher bioavailability of and! Modalities that are designed to cope with emerging challenges in intracellular applications, e.g chemical dispersant the... Dechorionating ( removing the shell ) from 1 day old zebrafish embryos and larvae the of... Schmid, B., Schmied, C., Fahrbach, F. O K Tazzyman. 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Exposure of zebrafish embryos, downstream gene expression can be set up in a breed - ing tank a... Aggregated into the biggest particles around the membrane protein and then caused a little toxicity to of... Family of proteins during early zebrafish development History, and Xenopus Biology with Stereo.!

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